Chapter 100: Delicate Thoughts (Asking for Subscription)

While running the experimental process, Luo Tingzhu's thoughts were slightly, just a little bit, scattered; Luo Tingzhu felt it was at most just a little bit.

However, Luo Tingzhu hurriedly suppressed her own thoughts and hurriedly continued running the process.

When the experiment finally reached a stage where it could wait, Luo Tingzhu temporarily left the laboratory, and also carefully made a label: "Please Senior Brothers and Senior Sisters don't touch! ~ltz"

This is a kind of politeness, to avoid others thinking no one is operating here, or that it was forgotten to be turned off, and then accidentally throwing things away.

Such things have happened in the laboratory before, which is why there is such a reminder.

Luo Tingzhu walked out of the laboratory, and when walking towards her own SmallDesk and SmallStool, she happened to hear Fang Ziye's fellow trainee Jie Han pestering Fang Ziye: "Senior Brother, why do we need to add this reagent in this step?"

"That is called Dimethyl Sulfoxide (dmso), it is an important permeable cell protectant."

"When cryopreserving cells in deep low-temperature liquid nitrogen, to prevent damage caused by intracellular ice crystal formation, osmotic pressure changes, and cellular structural disorder during the freezing process, a freezing protectant containing dmso must be used. dmso can rapidly penetrate the cell membrane and enter the cell, lower the freezing point, delay the freezing process, and simultaneously increase intracellular ion concentration, reduce intracellular ice crystal formation, thereby reducing cell damage." Fang Ziye answered with slight patience.

"Then what if we don't add it? What would happen? Or what if we add other reagents?" Jie Han was like a curious baby.

Fang Ziye hadn't considered such a question: "I don't know either. Currently, our Country should still not have made original progress and breakthroughs in cell cryopreservation technology. Currently in medical cell experiments, the freezing step all uses dmso."

"Jie Han, were you also like this before, not reading any books and just asking directly?" Fang Ziye asked one more question; this Jie Han really had a lot of questions after coming to the laboratory.

"No, I read books before asking. For example dmso, molecular formula c2h6os, colorless odorless transparent liquid at room temperature, a hygroscopic flammable liquid. It has high polarity, high boiling point, good thermal stability, non-protic, miscible with water, can dissolve in ethanol, propanol, benzene, chloroform and most organic substances, known as the 'universal solvent'..." Jie Han immediately started reciting from memory.

"It's just that from its biochemical function, I couldn't find any effect that protects cells; on the contrary, I know this is a component of pesticide. You tell me, Senior Brother, a pesticide component, how can it have no harm to cells?"

"Also according to literature, dmso is also a permeability protectant, able to lower cell freezing point, reduce ice crystal formation, reduce free radical damage to cells, and change biological membrane permeability to electrolytes, drugs, toxins, and metabolites."

"Then you tell me, can such a thing be applied clinically?" Jie Han was just a question baby; every question was very tricky.

"Did you also often ask questions like this during your undergraduate years?" Fang Ziye asked.

Jie Han thought for a moment and replied: "Hmm, many of our teachers were asked until they were impatient, so they would occasionally bestow some research projects on me, feeling I had a tiny bit of talent."

Jie Han answered seriously, not feeling this was a shameful thing at all.

Not understanding is why I ask; if I understood, why the hell would I ask you?

But Jie Han's questions were basically all things ordinary people wouldn't understand, and were all questions with research value; moreover, his range of knowledge was quite broad, thinking about problems from both biochemistry and traditional chemistry perspectives.

"Senior Brother, here..."

Fang Ziye was speechless; he didn't expect Jie Han was just doing a cell experiment, copying the cell experiment process, and seemed to bring ten thousand whys with him.

In the distance, Luo Tingzhu, who had secretly glanced at Fang Ziye, couldn't help but slightly prostrate her body, pursing her lips to hold back laughter, guaranteeing she wouldn't show teeth while marking checkmarks one by one in her ExperimentNotebook, just to mark which step of operation and experiment had been done and which hadn't.

Immediately after, Luo Tingzhu's train of thought drifted a bit far again, mainly because she was very formally thinking about that sentence Jie Han said just now in the Cell Room, which made her thoughts slightly uneasy.

To tell the truth, in the five years from undergraduate enrollment to now, Luo Tingzhu had seen quite a few Senior Brothers who were good to her, some relatively excellent Senior Brothers, to the point of numbness.

Senior Sisters, Senior Brothers, teachers, and classmates who praised her for being pretty and good-looking were countless.

The reason she chose Orthopedics Department was because Luo Tingzhu felt the Senior Brothers in Orthopedics were better; here she had a certain sense of security. Plus, during her previous internship, she discovered that although Orthopedics had the highest medical dispute incidence among surgical departments, it had the lowest limb-related medical dispute incidence.

In addition, during her internship, some unpleasant events occurred in Internal Medicine; she resolutely chose Surgery, after all most surgical departments would not choose the approach of settling things quietly.

When Luo Tingzhu first came, she came looking for a good teacher. Among the five sub-specialties, she found Trauma Surgery's competition was relatively the smallest, so she came to find Teacher Deng.

The Eight-Year Program directly selects a Supervisor; as long as the Supervisor agrees, admission is no problem, it doesn't occupy any quota, and it's all free choice. Generally speaking, PhD Supervisors also wouldn't disagree to accept Eight-Year Program students.

Even though Deng Yong's Group's funding was tight, Teacher Deng still accepted Luo Tingzhu as a student. After coming here, Luo Tingzhu felt a long-lost sense of security.

Whether it was the Senior Brothers before willing to stand up for her and block those 'rich and powerful' people bothering her, or the Orthopedics Senior Brothers willing to give her more opportunities, Luo Tingzhu was still quite moved.

Of course, such moving moments don't only exist in Orthopedics, and aren't only from Fang Ziye treating her so well.

Speaking of people good to her, even among the Orthopedics Senior Brothers, Fang Ziye can't compare to Shaoqing Senior Brother, and even less to Second-year Doctoral Student Big Brother Gu's meticulous care.

But Fang Ziye has one unique point: he is currently the only one among all peers and Senior Brothers Luo Tingzhu has met who has better talent than her and progresses faster than her.

Not only experimental talent, but also clinical professional operation capability.

To the extent that at the beginning, in the Training Room, Luo Tingzhu often faced Fang Ziye with the identity of a Senior Sister, merely adopting a Junior Sister's tone, tirelessly pointing things out to Fang Ziye.

Although she called Fang Ziye Senior Brother.

But such time didn't last very long; Fang Ziye suddenly caught up all at once, and then suddenly, fiercely ran to the front.

Another aspect: Luo Tingzhu also knew that in Deng Yong's Group, she definitely wouldn't have enough experimental funding when first entering; it all relied on the Senior Brothers' support.

For example Big Brother Gu, for example Wang Yuanqi Senior Brother, even Yan Zhiming Senior Brother who was admitted early by Teacher Deng, would all give Luo Tingzhu some reagents, operation Equipment, and such.

But at this time, Fang Ziye showed nothing; in the end, it was still her begging Teacher Deng, hoping Teacher Deng would speak to Teacher Yuan, because Teacher Yuan's research group still had some spare money.

She just wanted to do experiments together with this Senior Brother Fang Ziye who had good operations and high basic cell experiment success rate, counting as familiarizing herself with the basic cell experiment process.

To strive for certain research funding for herself; the current result is, Luo Tingzhu succeeded, and she would feel she was actually a bit impressive—

Although, some key experiments were done for her by Fang Ziye; Fang Ziye was willing to help.

But actually these things, other Senior Brothers could also do; just the success rate wasn't as high as Fang Ziye's.

A person follows the virtuous and their character naturally rises; if the opportunity is suitable, no one would mind learning together with more excellent people, finding better teachers, finding higher-paying jobs.

To save money, avoid waste after failure leading to insufficient early experiments, causing success rate to drop when applying for projects, she had Fang Ziye help several more times, and also conveniently borrowed some of Fang Ziye's experimental processes to accumulate experience for herself, only then reaching now, where she can operate all basic cell experiment processes relatively proficiently in just a few months.

Teacher Yuan didn't express that she could use his project funds for experiments; Luo Tingzhu also completely didn't mention this matter!

To her, even parents are hard to ask; how much more so others?

Slowly do it forward; doing basic cell experiments has never been achieved overnight.

For a long period, Luo Tingzhu thought she might be able to walk ahead of Fang Ziye in getting projects; after all her Supervisor was a Chief Physician, a Professor, which could still maintain a bit of leading advantage.

But this time, although Luo Tingzhu got a School-level Project, in the City-level Project aspect, the project she applied for didn't win the bid; instead Fang Ziye won the bid, which made Luo Tingzhu completely baffled.

Around November, Luo Tingzhu felt she might have encountered an 'Expert' of the same level as herself; Fang Ziye Senior Brother, previously always unknown, actually lacked a 'Trigger Point'; once it erupted, all potential erupted out.

At that time, Luo Tingzhu still occasionally maintained a battle desire with Fang Ziye.

Benign competition, mutually comparing capability improvement; this is a good mindset.

Early December, upon learning Fang Ziye's Incision Technique had reached the Training Room's passing requirement even further than herself, Luo Tingzhu's first reaction was disbelief; second reaction was that her talent actually wasn't on the same level as Fang Ziye; Fang Ziye was too excellent.

From then on, Luo Tingzhu did one thing many people couldn't detect: slowly paying attention to Fang Ziye's work and experiment state, including sometimes in the Training Room she would sneak a peek at what Fang Ziye was doing.

The things done were all the same as hers, conventional and by the book.

But just this kind of attention shocked her incomparably; finally she matched up the nicknames the Old Professors gave Fang Ziye.

Step by Step High, NoNeedThisName.

For instance, recently with Hemostasis Technique, Luo Tingzhu watched closely as Fang Ziye went from forty-three times in one minute, forty-four times, forty-seven times, all the way to fifty-one times, with the occasional fifty-five times.

This time, merely one month, she herself would need at least two months or even longer……

And such prolonged attention caused Luo Tingzhu to occasionally experience mental turmoil, even occasionally when seeing Fang Ziye, her mindset was not that calm.

Then came the past few days, Luo Tingzu practiced hard for a long time, finally felt she could match Fang Ziye in Incision Technique and pass the test, planning to quietly perform.

Fang Ziye called people over in advance, her performance truly became quiet, and in the end it was still Fang Ziye who discovered something off with her incision practice material, had someone call her back, letting Teacher Deng see her progress.

Interacting with excellent people requires adopting the mode of interacting with excellent people.

At the beginning, Luo Tingzhu treated Fang Ziye as a Fellow Trainee, thus with a mentality of giving guidance; later treated him as a classmate, 'SameKind', so Luo Tingzhu would send her grant applications etc. to Fang Ziye for revision, this counted as MutualExchange.

Luo Tingzhu privately also did a lot of literature exploration on cutting-edge research of Giant Cell Tumor of Bone and corresponding pathways, including some LiteratureReserve on upstream and downstream pathways of hk2 that Fang Ziye is currently doing.

The plan was, if Fang Ziye came to ask, she could return the gift at any time.

But Fang Ziye delayed and didn't send the grant application over, she thought ah, Fang Ziye cannot apply for research projects himself, so he didn't have such thoughts and preparations.

That's fine, NextYearAgain.

Next year Fang Ziye can apply for research projects himself.

Holding such a mood, Luo Tingzhu then suddenly discovered, Fang Ziye never thought of letting her help at all; after learning from Teacher Deng that the City-level Project was taken by Yuan Weihong, Teacher Deng completely didn't care, because he looked down on it, but Luo Tingzhu instead had another small breakdown.

She wondered if Fang Ziye looked down on her capability?

But later, Luo Tingzhu slowly discarded such unilateral thoughts; it's not that Fang Ziye looks down or not, but Fang Ziye has no need to help, because Fang Ziye has already done preliminary research to a depth……

Where improper operation is possible, letting his entire research integrity quality collapse.

Of course, the research integrity here is not fake, but need money.

Clearly you're almost done, yet you still need money to say you want to do such a project, isn't this another kind of breach of trust?

Naturally, such a thing, Luo Tingzhu would never whistleblow; Fang Ziye saved money from his own previous project funds, scraped it out, does his own experiments, what's the problem?

NoProblem. Everyone in the lab knows a principle: SaveIfCan, CannotSaveThenNot, but Fang Ziye's stinginess and saving degree is even higher than hers.

This made the always carefully calculating Luo Tingzhu even MoreCurious……

Unknown time passed, Fang Ziye suddenly said: "Little Jie, let's stop our Q&A session here today, your thinking is too jumpy, your Senior Brother can't keep up."

Fang Ziye admitted defeat, slightly raised volume.

Jie Han's whole person as usual, the mole at the corner of his right eye flashed and blinked again: "Oh, okay Senior Brother."

Such an answer, quite regretful, interest unexhausted.

Fang Ziye then asked: "Do you usually ask Lan Tianluo questions like this too?"

"Mhm, Tian Luo has lots of questions too, he even asked why White Blood Cell Count is to the sixth power rather than fifth power such questions." Jie Han nodded.

Fang Ziye could only stick up a pair of big thumbs.

Then QuickEscape: "Tingzhu, is your experiment almost done? Want to go watch together?"

Luo Tingzhu heard Fang Ziye ask this, her spirit returned to position again.

Nodded while checking time: "Senior Brother, OneHourLeft."

"Oh." Fang Ziye felt very regretful, checked time again, then looked at his Fellow Trainee Jie Han, moved a seat, truly was scared by Jie Han's questions.

Through this point, Fang Ziye couldn't help but suspect, the reason Jie Han still hasn't followed Yuan Weihong for a second Outpatient Clinic until now, perhaps is this reason.

Once, that time was August, Jie Han just enrolled, Fang Ziye felt Fellow Trainee just came, gave Jie Han a chance to follow Outpatient Clinic, let Yuan Weihong bring Jie Han along, this counted as a courtesy.

Yuan Weihong also agreed, felt should treat new Disciple better, but from then on, Yuan Weihong never mentioned this matter again.

Fang Ziye then opened his Laptop, started drawing again.

Moreover, Fang Ziye currently has many images, need to classify and organize one by one, BuildFolders these, including OriginalImage, OriginalData etc., all need to be classified one by one.

One big Folder contains over ten small Folders, one small Folder contains seven or eight Folders, then reaches the data storage step.

Equivalent to hundreds of workload results, just basically, except hk2 and Giant Cell Tumor of Bone have formed a ClosedLoop from cell experiment angle, the others are still OpenLoop steps.

Fang Ziye is just afraid sometimes his brain gets hot, what if when writing grant application he puts all results up, the project Reviewer sees, you've already done everything, why apply for project funding?

At this time, Luo Tingzhu sitting in the distance, also secretly perfecting the data presentation section of a new Meta-analysis she wrote.

Just typing speed very slow, thinking in heart, every time Senior Brother Fang invites her to do experiments and participate in experiments, the tone is very sincere, so it shouldn't be that he looks down on her capability.

Or perhaps it's enthusiasm, after all Senior Brother Fang is very willing to participate in all Senior Brothers' projects, as long as there's free time, if a Senior Brother is doing experiments, he's willing to participate.

Don't think wildly, do more, think more, think less wildly, oh no, must not think wildly.

Luo Tingzhu then started thinking wildly again.

Until one hour later, Fang Ziye like a timed Alarm Clock, actively invited Luo Tingzhu to go participate in the later experiments together.

Luo Tingzhu's heart slightly panicked, accidentally deleted One Line, hurriedly used undo button, undid that sentence, then said: "Senior Brother, wait a moment, let me finish writing this sentence."

Luo Tingzhu finished speaking, wrote the words inthehipreplacement, then looked left and right, then clicked the File save button.

Stood up started tearing a new pair of LabGloves, Walking Side by Side with Fang Ziye into the Laboratory.

(Chapter End)