Chapter 116: What the Heck? I've Got Something On, Leaving First (Asking for Subscriptions)

Fang Ziye started eating first, so he also finished lunch first.

After eating, he stood up, threw the lunch box into the trash can at the stairwell, and used his fingerprint to open the laboratory door.

At this moment, a Senior Brother Liu, whom Luo Tingzhu had invited from some discipline's laboratory, was scrolling on his phone, the picture of effortless mastery; seeing someone open the door, he looked up, and he and Fang Ziye exchanged a pursed-lip smile, counting as a greeting.

Fang Ziye didn't know him; at least he wasn't an Orthopedics senior brother, so Fang Ziye didn't go up to say anything either.

Luo Tingzhu finding someone to ask questions was naturally perfectly fine.

This senior brother's brave and direct initiative wasn't problematic either.

As long as he had no ill intentions, no crooked schemes, Fang Ziye stood at no angle to interfere with anything; he just needed to do his own job well.

Naturally, Luo Tingzhu hoped Fang Ziye could show off a little; from her angle, she hoped this Senior Brother Liu wouldn't come bother her again; this point, Fang Ziye absolutely would not agree to, nor would he take the initiative to provoke; just do his own job well.

Otherwise, if he ran into a hard nut, he'd end up making a fool of himself — wouldn't that be a laughingstock?

Quietly do his own job well, demonstrate a certain capability.

Coming to the Orthopedics Laboratory to help with experiments was welcome; but if it was about swaggering and throwing weight around, that was out of the question.

Even if he could fight again, if Yuan Weihong or Qin Geluo were called, he could only slink away in disgrace.

Naturally, Teacher Deng's hands-on skill in basic experiments might not be that great, but there's still Associate Professor Hong Zili!

Associate Professor Hong Zili's research foundation cannot be directly compared with Professor Liu Huanglong of Hand Surgery, but in Associate Professor Hong Zili's own specialty field, he is currently at the Academic Giant level, even more formidable than Liu Huanglong; it's just that his professional ability isn't that strong; he belongs to the absolute top tier of surgical research at Zhongnan Hospital.

Even a Chief Physician professor like Teacher Deng can have no students, but Associate Professor Hong Zili cannot lack Master's and Doctoral students; this was Teacher Deng's own original words.

Fang Ziye needed to use the cell operation bench, and Luo Tingzhu likewise needed to use it.

Moreover, Luo Tingzhu's cell operation bench was reserved by Fang Ziye for her; the time was only a dozen minutes later than Fang Ziye's.

There were three operation benches in total inside the cell operation room.

Fang Ziye entered fully geared up, took out his culture medium and PBS buffer pre-warmed earlier than Luo Tingzhu's, then also the heatable reagents and non-heatable reagents, sprayed each with alcohol for disinfection, and placed them into the operation bench.

By the time Luo Tingzhu brought Senior Brother Liu into the laboratory, Fang Ziye had already started operating.

The alcohol lamp in the operation bench was lit, the exhaust fan turned on, the glass of the cell operation bench pulled down, and Fang Ziye began trypsin digestion on the cells inside the culture bottle.

Trypsin digestion is a step that many operations must go through.

When cells are in the culture bottle, they grow adherent; they need to be digested to suspend the cells, only then can centrifugation separate cells from reagents, then proceed with passaging, aliquoting, transferring to culture dishes and culture plates; it's equivalent to a procedural operation.

This operation can be analogized to stepping out your front door from your usual residence; whether you go downstairs to eat, buy fruit, or do other things, you first have to step out the door.

This step is very simple, not much operation to speak of; the main credit all belongs to the centrifuge.

Fang Ziye aspirated the trypsin suspension to avoid over-digestion killing the cells.

Because adherent cells are like elementary schoolers sound asleep in a dorm; trypsin is like throwing a smoke grenade into the dorm.

But the smoke grenade only makes them come out the dorm door, not suffocate them to death inside.

However, after Fang Ziye had completely suspended all the separated cell groups and re-configured the ratios.

Fang Ziye hurriedly performed a counting process on the suspended cells first.

The cell counting operation is temporarily not in the cell room; you have to go mix some reagent, run the flow cytometer, and determine the concentration.

Cell concentration within the same batch of samples can be considered equivalent.

Just like drinking a bottle of beverage, the first sip and the last sip should theoretically have equivalent sweetness or taste.

But after counting, Fang Ziye didn't bring the samples back; instead, right there, he directly sealed them and threw them into the biohazard bag.

But knowing the concentration, Fang Ziye's operation began.

Diluting samples is actually simple in principle: just add 1ml of sample into 99ml of water, and you've diluted a hundred-fold; if you add 1ml of sample into 999ml of liquid, that's a thousand-fold dilution.

And when doing serial dilution experiments, you need to repeat this operation once; generally each time is a 10-fold dilution.

According to normal operation, you actually only need to prepare the liquid volumes in advance: 9ml, 99ml, 999ml.

Then add 1ml of cell suspension into each, mix separately, then aspirate 1ml from the 9ml+1ml mixture and transfer into the 99ml.

At most it's just a matter of changing a few pipette tips.

But today's Fang Ziye did not operate this way.

He set the pipettor to 200ul, then directly dispensed 1ul, 10ul, and 100ul amounts into the culture dishes.

No scale adjustment, no pipettor change.

This kind of operation is absolutely Yuan-style operation.

After all, this is equivalent to blind operation; to non-quantitatively squeeze out 1ul of liquid under a 200ul pipettor — isn't that talking nonsense?

But Fang Ziye just did it like that.

Naturally, these slightly detailed issues were not seen by Senior Brother Liu and Luo Tingzhu operating next to him; they only saw that Fang Ziye didn't even change the pipette tip, just pecking away like a chick pecking rice, tap tap tap.

After tapping for a while, Fang Ziye then added 999, 990, 900ul and other base-amounts of pure culture medium liquid to the different samples respectively.

Then added a few more test tubes, and re-used the most standardized operation to continue diluting cell concentration.

This time, Fang Ziye was to find the optimal cell concentration order of magnitude for operation, finally choosing the most suitable concentration for the experiment.

After all, the relationship between this miRNA and HK2 in giant cell tumor of bone that Fang Ziye is currently doing has not been done by anyone else; Fang Ziye referenced the optimal concentrations of other tumor cells' relationship with HK2, couldn't get the best images, so Fang Ziye could only go find them himself.

Everything is unknown, even the parameters are unknown; this is the difficulty of some basic experiments.

But it doesn't cost much, just takes time...

After Fang Ziye operated like this a few times, he first put the lid on a twenty-four-well cell culture plate, then sent it into the constant temperature cell incubator.

Fang Ziye's next operation was to handle the concentration gradient difference of another giant cell tumor of bone cell line.

Because different cell lines' optimal response concentrations might still be different. This is like clinically, same giant cell tumor of bone, but because classification differs, the neoadjuvant chemotherapy regimens pre- and post-operation also differ.

All are groped out.

The experiments Fang Ziye does, he wants to target for publication not some ordinary journal, at least a high-quality journal above 15 points, so naturally he must make the most complete, most thorough preparations.

And, after finding the order of magnitude, Fang Ziye still has to find the optimal response pre-parameter of 1-9, to see if there are differences under the same order of magnitude.

And generally once you reach the 1-9 optimal parameter, plus the order of magnitude's optimal solution, there's no need to search further.

The giant cell tumor of bone cell lines Fang Ziye operated on included gct-0404, gct1, and gctsc. (Note: These cell lines are purely original; please do not correspond them with reality, knowledgeable readers.)

After Fang Ziye left, Senior Brother Liu, who had been watching, looked at Luo Tingzhu thoughtfully and asked curiously, "Junior Sister Luo, is this your Senior Brother or your Junior Brother? His experimental operations are this fierce?"

"I saw just now he seemed to be preparing to do a standard curve operation, and he just went at it blindly?"

Fang Ziye's operations, in the eyes of those who don't understand, are merely adding things, but in the eyes of professional researchers doing cell experiments, what on earth are these?

If your teacher saw this, they could strip your skin off.

When you could have a standard operational path, you don't take it; you insist on creating difficulty for yourself.

However, according to experience, generally speaking, 1ml of water is 20 to 25 drops, so one drop of water is 0.04 to 0.05ml. If using a regular dropper, it is approximately 0.05ml; if using a burette, it is approximately 0.04ml.

"Lao Liu" himself had never calculated how many ul a drop from a 100ul pipette tip would be, but regardless, if his own student dared to operate like this, he wouldn't just give them a slap; he would definitely scold them into autistic withdrawal for two or three days, making them roll out of the lab to reflect properly.

"Oh, Senior Brother Fang? Don't know." Luo Tingzhu snickered inwardly, her expression serious.

"Senior Brother Fang's operational proficiency is much better than mine. When I learned with Senior Brother Fang before, he always operated by the book, but recently I'm not too sure."

Senior Brother Liu didn't say anything more. After finishing the cell aliquoting process, he took Luo Tingzhu to construct the miRNA silencing cell line.

This requires a certain waiting period.

Slightly boring.

Or perhaps because Luo Tingzhu had chattered in Senior Brother Liu's ear for a while, Senior Brother Liu got a bit curious—what kind of ghost was this Fang Ziye, who just now made him unable to understand?

So, after coming out with Luo Tingzhu, he asked, "Junior Sister, can you show me around casually? I haven't been to your Orthopedics laboratory yet."

Seeing the laboratory's scale wasn't particularly large, slightly smaller than the Urology laboratory he was in, Senior Brother Liu made this proposal.

Luo Tingzhu didn't refuse. A guest is a guest; whether they came thick-skinned or however they came, as long as it wasn't forced, she had to do a certain amount of reception work.

Senior Brother Liu was an outsider to the laboratory, brought in by her, so she couldn't let him wander freely; she had to follow.

Senior Brother Liu didn't chase after Fang Ziye to look, but walked around looking to see if he could meet an acquaintance. Unexpectedly, he really did meet one—his classmate, currently a doctoral student in Thoracic Surgery.

Named Qian Qiaofeng, nickname Helpzhu (Helpzhu (Bangzhu)).

Qian Qiaofeng was tall and thin, with a handsome face, also wearing glasses. He happened to run into Senior Brother Liu and Luo Tingzhu as he was going out.

Seeing Luo Tingzhu standing with hands behind her back beside Senior Brother Liu, as if understanding something: "Yuan Ge, are you here for an inspection? Or a visit?"

Liu Tengyuan is Senior Brother Liu's name.

"Helpzhu (Helpzhu (Bangzhu)), you? You're in this laboratory?" Liu Tengyuan's mood instantly tightened upon seeing Qian Qiaofeng.

Qian Qiaofeng is a doctoral student in Thoracic Surgery, a professional-degree graduate student, but his experiments are also extremely formidable. Currently, among their cohort of doctoral students, Qian Qiaofeng is the evergreen of the National Scholarship.

To get the National Scholarship, it's not an internal professional evaluation; all medical students from internal, external, gynecology, pediatrics, dermatology, psychiatry, etc. are pulled together for evaluation. Only those close to the top ten percent have a chance to get the National Scholarship. Basically, every year requires quite a lot of output.

"Mm, my Master does experiments here!"

"Tingzhu, will you introduce, or should I let Yuan Ge introduce?" Qian Qiaofeng set aside the tray holding experimental instruments and reagents and started chatting.

Luo Tingzhu hurriedly explained: "Senior Brother Qian, this is Senior Brother Liu. He came to help me with experiments. He wants to visit our laboratory."

Qian Qiaofeng's gaze slightly converged, his look at Liu Tengyuan carrying contemplation.

When Liu Tengyuan saw Qian Qiaofeng, his gaze was slightly cowardly. This damn thing, how did he just happen to barge into the laboratory of Hong Zili, the thoracic surgery research big shot? Isn't this ramming headfirst into an iron plate?

Liu Tengyuan hurriedly explained: "Helpzhu (Helpzhu (Bangzhu)), you're not thick-skinned/kind. Your Junior Sister has difficulties, you don't help, and instead call me from downstairs."

"Can't you read less manga and anime?"

Qian Qiaofeng turned sideways holding the tray: "I watch a ghost. I haven't followed anime for two weeks. Still watch anime."

"Busy to death……"

With this back and forth, Luo Tingzhu was instead relieved. Qian Qiaofeng and Liu Tengyuan were classmates. Since Liu Tengyuan wanted to look, Qian Qiaofeng couldn't refuse the face, and personally took Liu Tengyuan around.

Liu Tengyuan secretly suffered bitterly while secretly hating this Qian Qiaofeng for being simply not thick-skinned/kind, or not caring about human relations. Do I need you to take me around?

But after a while, as Qian Qiaofeng slowly introduced some of his Senior Brothers and Junior Brothers to Liu Tengyuan, Liu Tengyuan's expression gradually became serious and calm.

"Damn, the equipment in this laboratory is impressive. Compared to a general provincial key laboratory, it seems even more comprehensive. This cell storage refrigerator—there are actually this many?"

"It's okay. Temporarily placing is enough, but sometimes it's still not enough. There are many people in the lab, too many things."

"Look, this cabinet belongs to my Senior Brother. One person occupies half of it, over ten different cell lines……"

"And this one……"

Liu Tengyuan nodded: "Professor Hong is still formidable. Can he be promoted to Professor this year?"

"Probably difficult. My teacher put most of his energy into research. He couldn't pass the Chief Physician step. If he can be promoted to Chief Physician this year, then he can be promoted to Professor." Qian Qiaofeng explained just like that.

Then the two walked to an operating room. Qian Qiaofeng immediately paused: "This one is a student of Professor Deng from Orthopedic Traumatology, our Junior Brother. Simply fierce as hell!"

"Basic experimental operations, even more mature than my Senior Brother by a little bit."

"Look, what do you think the success rate of all his current operations is?"

"Success rate eighty percent?" Liu Tengyuan gave a rather exaggerated number.

Many people doing experiments might not get results in one go, needing to repeat two or even three times, constantly adjusting.

"Currently maintained at ninety-eight percent. At least fifty-plus small operations done, only repeated once so far. This ninety-eight percent was calculated over ten days ago."

"Old awesome. My Senior Brother said, after he graduates, if I can't handle any experiment in the future, I can find him for help."

"That one, also Orthopedics, Dong Wenqiang, our cohort, Academic PhD. You might not have much contact with him."

"He's a relatively reclusive person. Could have gotten the National Scholarship, couldn't be bothered to participate. Family doesn't lack this bit of money. Just wants to work honestly. Published more articles than me. Two articles above six points during PhD. Currently estimated one more, being submitted outside."

"This one is……" Qian Qiaofeng knew them like the back of his hand.

After walking roughly a circle, Liu Tengyuan collected his embarrassed mood and hurried back to Luo Tingzhu's side.

Couldn't leave immediately either. Had promised to help Luo Tingzhu finish the experiment, so could only bite the bullet and do it.

However, after he finished handling the cell line cell strain operations, Liu Tengyuan hurriedly said: "That thing, Junior Sister, operations here are about done. Senior Brother still has some matters. I'll leave first."

"Thank you, Senior Brother. Next time if Senior Brother needs help with experiments, you can call me." Luo Tingzhu hurriedly stood up and saw him off a distance, speaking politely.

Before, she might have said to treat to a meal. But now, she wouldn't treat Senior Brothers to meals. Because this kind of meal isn't good to eat. She can't pay the money herself, instead owes a favor. Not worth it.

Liu Tengyuan thus hurriedly left carrying his Armani backpack, not daring to linger any longer.

However, Luo Tingzhu paused several times on the word "backpack" in Liu Tengyuan's case, then pursed her lips and turned away; as she turned, Luo Tingzhu was still muttering in a low voice.

"Dad said, when dating, don't look for someone whose family conditions are too good; finding someone about the same is more suitable. Best to find someone whose family background has a card balance ten to twenty times the pocket money; too high makes true sincerity unlikely, too little makes life difficult."

"For general people, a bag they'd buy without blinking might be between one-thousandth and one-ten-thousandth of all their family assets. Young people might be a bit more showy..."

"But this card has eight hundred fifty thousand; that also seems not so easy to find."

"Forget it, ignore it..." Luo Tingzhu walked quickly toward the cell room, seizing the time to take photos.

(Chapter End)