Chapter 48: Originally No Need to Be Friends (Asking for Favorites, Asking for Follows)

"These, were they done by you?" Yuan Weihong pointed at the label fzy1016 on the six-well plate and asked.

"Mhm, Master, should be, no problem, right?" Fang Ziye was currently tidying his mask at this moment.

The teacher summoned him, so Fang Ziye could only rush to the laboratory with all speed, not daring to delay in the slightest. At this moment, he didn't even have time to check whether his slippers were on correctly.

Mhm, entering the cell room, in principle, you cannot wear your own shoes; you need to buy a separate pair and leave it on the shoe rack outside the cell room for use.

Yuan Weihong examined it a few more times, then continued focusing on the microscopic view: "First time doing it, and you already did it to this extent?"

"A genius, huh?"

"Who helped you do it?" Yuan Weihong's thinking jumped to that conclusion first.

"Master, this really was done by me myself. The Senior Brothers in the lab, how could they have time to help me? Though Junior Sister Luo Tingzhu did give some pointers. But she didn't help." Fang Ziye had already guessed his operation shouldn't be bad, so he felt at ease.

Just looking down at his feet, it seemed this pair of slippers really wasn't his.

Looking toward the doorway, Fang Ziye discovered a head peeking through the cell room door frame, looking inside — it seemed to be Luo Tingzhu.

Fang Ziye was helpless, so he could only extend his foot.

Just now he came too urgently; he thought his scratch assay cells had a problem and the teacher found out, so he grabbed a familiar-looking pair of slippers without even checking the small name label.

The slippers in the lab are basically all alike; if you don't look at the name label, there's no time to recognize them.

The peeking head instantly shrank back.

Yuan Weihong also noticed Fang Ziye's small movement, thought carefully for a moment: "You wore whose shoes?"

"Your shoes are on my feet." Yuan Weihong had also hung around the lab before, so he naturally knew the corresponding rules.

Fang Ziye swayed left and right a few times: "Master, it's fine."

"Scratch assays are generally photographed at 0, 6, 12, 24 hours. Why didn't you follow the standard?" Yuan Weihong turned his head and asked back.

This was also his purpose in calling Fang Ziye here. October 16th was yesterday; Fang Ziye obviously hadn't properly observed and photographed.

"Master, Giant Cell Tumor of Bone grows relatively slowly, so isn't it okay to delay photographing?" Fang Ziye asked.

"You say it grows slow so it grows slow? Are the images published in papers all the original raw materials?"

"If you don't come, you just wait for someone else to clean up for you? Even if it's blank, invalid, meaningless data, is that not experimental data anymore?" Yuan Weihong seemed to have found a chance to lecture, and pressed on.

Fang Ziye immediately wilted a little.

On this point, Fang Ziye really hadn't paid special attention; mainly he read other literature related to Giant Cell Tumor of Bone, and the records inside were all 24h, 48h, 72h observations at 24h or 12h intervals, not six-hour or absolute 12h data records.

Without cognitive experience, even if you add points and operational proficiency improves, you'll still suffer losses.

"Master, I was wrong, I'll redo it once more." Fang Ziye was also honest; knowing his mistake, he admitted it.

"Still wait for you to redo it, does it not cost money?"

"The money the Country gives for research grants, is it for you to fail whenever you want?"

Yuan Weihong continued lecturing: "Speaking small, it's doing things without rigor; speaking large, you're wantonly squandering the Country's financial grant resources!"

Yuan Weihong carefully collected the three six-well plates, then handed them to Fang Ziye, letting him disinfect the surface first, then put them into the cell incubator.

Fang Ziye was very obedient then.

But after Fang Ziye finished placing the six-well plates, he said: "When there's a chance, treat Luo Tingzhu to a meal. She's a young girl, for our group's little cells, she stood guard this long."

"Thank her properly."

Fang Ziye's head subconsciously tilted; this was a habitual micro-movement when Fang Ziye suddenly thought.

"Transwell cell invasion assay, do you know how to operate?" Yuan Weihong, while walking toward the door, asked next.

Fang Ziye nodded.

"For real or fake?" Yuan Weihong took off Fang Ziye's lab slippers, his movement pausing slightly. Obviously Fang Ziye's answer stunned him.

The reason he came to the lab today wasn't specifically to supervise Fang Ziye's experimental operation; it was because this little girl Luo Tingzhu asked him for advice. Mainly Luo Tingzhu asked around and no one had time to teach her, so she asked Yuan Weihong.

Yuan Weihong also had a deep impression of this young girl Luo Tingzhu, so he thought he'd drop by when free, and incidentally check Fang Ziye's experimental progress. He discovered Fang Ziye's operation was exceptionally good, but had flaws.

"Should be about right." Fang Ziye nodded, tone steady, not daring to be careless.

"What are the experimental procedures?" Yuan Weihong didn't want Fang Ziye to wing it.

Criticism is criticism, carelessness is carelessness.

Damn, just entered the lab less than half a month, and directly matured to operating cell scratch assays. Such talent, looking nationwide, not saying there's none, but definitely not especially many.

After all, ordinary people, recognizing experimental equipment, copying experimental records, culturing cells and such operations, also need a period of time.

In Yuan Weihong's eyes, Fang Ziye had always been a diligent, reliable student, not a talent-type player.

Previously progressing step by step with slight improvement, that was accumulation leading to breakthrough, natural progression — not Fang Ziye making a huge leap. Now this sudden rapid progress, not long after entering the lab, truly stunned Yuan Weihong.

Between us master and disciple, who doesn't know who?

Little Antenna Baby.

"General procedure is cell culture and treatment — coating basement membrane — hydrating basement membrane — preparing cell suspension — seeding cells into upper chamber — cell fixation — cell staining — mounting — cell observation and counting." Fang Ziye replied slowly.

Although the reply was slow, it was precise.

"What about the experimental principle?" Yuan Weihong returned the shoes to Fang Ziye.

"Invasion assay is placing the chamber into the culture plate; inside the chamber is called the upper chamber, inside the culture plate is called the lower chamber. Upper and lower culture media are separated by a polycarbonate membrane. Upper culture medium is added to the upper chamber, lower culture medium to the lower chamber. Cells are seeded in the upper chamber. Because the membrane is permeable, components in the lower culture medium can affect cells in the upper chamber, thereby allowing study of the effects of lower culture medium components on cell growth, migration, etc."

"But tumor cells under starvation conditions will definitely strive to drill toward the lower culture medium. The number of tumor cells reaching the lower culture medium represents invasive capability."

"Same as high school: whoever is hungrier runs faster; everyone equally hungry, whoever runs faster eats first." Fang Ziye also made an analogy.

Yuan Weihong's mouth corner twitched; though blocked by the mask, his eyes showed struggle: "Like this you'll have no friends."

"Master, we are master and disciple, no need to be friends." Fang Ziye grinned, knowing he had passed this test and wouldn't continue to be criticized by Yuan Weihong.

"Teacher and friend — these four characters you don't take seriously, huh?" Yuan Weihong's tone was full of jealousy.

People who haven't soaked in the lab simply don't understand the hatred Yuan Weihong feels toward someone like Fang Ziye at this moment.

Go to hell, why don't you have to suffer the beating cell experiments give you? One try, one success; one try, one success. Are tumor cells your relatives?

(Chapter End)