Chapter 34: Pushing Open the Door Is Misty Rain in the Red Dust (Asking for Favorites, Asking for Follows)

Note: Some cell experiment pathways involved in this text differ slightly from real-world pathway guides, with three purposes: one is to avoid leaking the research project to the project group during filing, two is to avoid professional peers catching bugs, three is that this novel is mainly for entertainment, not a thesis collection. Please be aware, fellow readers.

Basically, for every medical basic researcher, the first step in pushing open the research path is cell resuscitation technique.

Medical basic experiments are experiments that, from the cell's perspective, perform various interventions on tumor cells or other cells, observing cell viability, function, proliferation and other characteristics.

The purpose is very simple: just hoping that one's own treatment can intervene in these cells, and possibly become a new direction for drug treatment of the disease type represented by those cells.

Can't just go straight at it on a human body!

"Cell thawing, washing, culture. Thawing, washing, culture." Fang Ziye first ran through the corresponding routine in his mind, then turned back and smiled at Luo Tingzhu.

But Luo Tingzhu did not mock Fang Ziye; instead she encouraged him, raising a fist toward Fang Ziye.

To do disease type research, you first need the corresponding disease type's cells. Teacher Yuan Weihong had already bought them, and after differentiation they were cryopreserved.

The start of cell experiments is to take a portion of the cryopreserved cells, resuscitate them again, and culture and proliferate them, to serve as subsequent experimental materials.

Generally it is liquid nitrogen cryopreservation.

Taking the cell sample from the frozen state, Fang Ziye immediately took the cryopreservation tube from the liquid nitrogen tank emitting white mist like a fairyland, and placed it into a preset 37°C water bath, gently shaking until the sample completely melted.

The faster this process the better, because the cryopreservation fluid contains some reagents that can lyse cells, unsuitable for cell growth; if the process takes long, it easily causes the cells to die.

If all the cell lines were killed by Fang Ziye, then Yuan Weihong would need to spend money again to repurchase.

Probably couldn't avoid a round of criticism.

Fang Ziye's speed was very fast; after thawing, he quickly put the cryopreservation fluid and cells into the culture medium, using the culture medium to dilute and wash the cryopreservation fluid.

This is like when a person is about to suffer gas poisoning, they must immediately go to a place with fresh air to breathe; detoxifying on one hand, avoiding continued toxin invasion on the other.

Because it was his first operation, Fang Ziye followed the best principle: he centrifuged the suspension after diluting the cryopreservation fluid with culture medium once, then poured out the suspension in the centrifuge tube, leaving only the adherent cell line, before re-suspending it evenly with culture medium.

Take the suspension, add it to the pre-prepared culture dish.

"Senior Brother Fang, you're so extravagant! If this were in the Internal Medicine Laboratory, you'd be scolded for such extravagance." Luo Tingzhu clicked her tongue slightly.

Even configured culture medium, because serum is added, is expensive; every milliliter can be measured in RMB yuan.

This one centrifugation by Fang Ziye probably wasted at least ten yuan.

Because a simpler, more crude method: after taking cells out of the cryopreservation fluid, directly dilute the cryopreservation fluid inside the cells with culture medium, or even directly add them into the culture medium.

"First time, waste a bit if you waste a bit, better than culturing the cell line to death."

"So after covering the culture dish, shake it for a few days, let it grow naturally for a while, after it's fully adherent and confluent. Then we can harvest cells, right?" Fang Ziye turned back and asked.

The cell lines Yuan Weihong stored away are all treasures, basically mother cell lines; they can be cultured for several passages and then used for experiments, no problem.

The price of this cell line is also considerable; Fang Ziye dares not waste it.

First culture one bottle, then cryopreserve a portion, experiment with a portion; his subsequent experiments will be done on the next few passages cultured from this mother line.

"Right, I thought Senior Brother Fang you wanted to just do it directly? After all Teacher Yuan has plenty of project funding, rich and imposing." Luo Tingzhu smiled.

Fang Ziye hurriedly shook his head.

"Junior Sister Luo, then today, have I already used nearly a hundred yuan?" Fang Ziye asked again.

Luo Tingzhu examined: "Culture dishes, plus Teacher Yuan's premium pipettes, one or two yuan each, plus culture medium, not quite a hundred, but seventy-something about right."

"Can't afford to provoke!" After spreading the cells evenly, Fang Ziye first took them to the microscope to observe.

After adjusting the focus, he found the cells in the field of view were all round, floating in the culture fluid, no distorted shapes, no motile bacteria; only then did he breathe a sigh of relief.

Just, at this moment, Fang Ziye observed the cell movement more carefully for a while, and suddenly discovered his knowledge points were growing wildly.

"+0.1!"

"+0.3!"

"+0.4!"

About half a minute passed, and Fang Ziye's knowledge points increased by 5 points at once.

This made Fang Ziye slightly stunned, somewhat baffled.

Luo Tingzhu had already left, and Fang Ziye was also wearing a mask, so his surprise and excitement were shared by no one.

He put the culture dish into the cell incubator, cleared the garbage from the cell operation bench, sprayed alcohol for disinfection again, took all his experimental equipment out, and returned them to the laboratory's refrigerator shelves and compartment drawers.

Only then did Fang Ziye sit again on a round stool in the laboratory, spacing out slightly.

In the distance, Luo Tingzhu was already prostrate, copying who knows what; farther away, some of Teacher Deng Yong's academic doctoral senior brothers were tapping keyboards, as if dissatisfied with the data they were processing.

Even farther, there were academic master junior brothers following a senior brother and senior sister Fang Ziye didn't recognize, going in and out of an experiment room, still asking: "Senior Brother, what do we do next."

At this moment, Fang Ziye could be said to have finally figured out some things.

In the clinic, those disease types one sees, those changes, have long since become very mature disease type diagnosis, treatment, and surgical plans and procedures.

But in the laboratory, one could say: any direction others have done, you cannot get a project approved; any direction that gets a project approved is a new direction and field.

Regardless of whether this direction has any fucking use, even if verification fails, that is still a failure no one else has verified.

What are knowledge points?

Knowledge points learned, right? At least Fang Ziye himself considers it so; treating and operating on clinical patients may yield subtle gains, and the knowledge brought by diagnosis and treatment is considerable, letting you truly experience this process, which inevitably has immature aspects.

But things in the laboratory, every experimental processing direction may be something no one else has ever touched.

For example, resuscitating this cell line myself — it was Yuan Weihong who specially used reagents to process it, seemingly a cell line with HK2 gene silenced; therefore, under such circumstances, even just observing its cell movement.

Is.

New knowledge.

Fang Ziye suddenly seemed to have found the gate to a new world, and beyond this gate is the human world, is misty rain.

What is knowledge?

The new, my own ignorance, unknown to anyone, the process from nothing to something — all count as knowledge...

(Chapter End)