Chapter 35

"Senior Brother Fang, can I borrow your PBS? Mine might be contaminated. My cells were growing perfectly fine last time, but after one PBS wash, they all died this time." Fang Ziye was deep in thought when Luo Tingzhu might have gone to check her culture dishes again at some point.

PBS buffer is the equivalent of giving cells a bath. When adherent cells are growing, after changing the original culture medium, they get washed once.

If the PBS buffer gets contaminated, that's bad news.

Compared to bacteria, tumor cells are just little Hello Kitties!

And once a cultured cell line gets infected, it hurts even more than getting injured yourself.

The little cells raised in the lab, and the corn and sweet potatoes that Agriculture PhDs plant in the ground, are the same principle. Extremely precious.

Fang Ziye was startled upon hearing this, turning to look at Luo Tingzhu with her slightly furrowed brows: "Junior Sister Luo, did you throw away the culture dishes? Can you let me take a look?"

Fang Ziye was like a curious baby.

It wasn't that Fang Ziye had never seen what contamination looks like, and Fang Ziye wanted to guess and see if looking at the contamination could increase his Knowledge Points.

If that were possible, this lab would be a massive treasure trove.

Luo Tingzhu nodded woodenly, also able to understand Fang Ziye's feelings: "What about the PBS?"

After contamination, finding the probable cause requires reordering. Luo Tingzhu might have prepared the subsequent experimental process, but now the cells were gone, equivalent to the experiment losing its subject. Wanting to go out for a meal, there wasn't a single restaurant that made sense.

"That's no problem, Junior Sister Luo, you take it and use it. Just seal it up after you're done." Fang Ziye hurriedly put on a sterile mask and cap, running into the cell room.

Reagents used inside the lab must be sealed with sealing film for storage after use, otherwise they are extremely prone to bacterial contamination.

Luo Tingzhu's PBS buffer was probably improperly sealed.

After Fang Ziye arrived at the cell room, he saw three culture dishes marked with the label ltz10.08, meaning Luo Tingzhu changed medium on October 08, or cells after re-passaging.

They were the subjects of the cell experiment, so others shouldn't use them randomly.

Fang Ziye's label was fzy10.10, making it convenient for everyone to distinguish.

Picking one up and placing it under the microscope, Fang Ziye indeed saw that the adherent cells had lysed where they should lyse, and in the suspension there existed various impurity debris.

Because the culture dishes were spaced apart, an oil lens definitely couldn't be used; bacteria couldn't be seen, and no other common contaminations like Burkholderia cepacia morphology were observed, so it could be guessed as bacterial contamination.

And looking like this, Fang Ziye indeed discovered, hey, his Knowledge Points had rapidly increased by more than 3 points.

Perhaps, the cell line Luo Tingzhu was currently culturing had also undergone special treatment. It was a special cell line with very few in number worldwide, at least lacking a mature theoretical system.

Such a cell line, even if contaminated to death, could let Fang Ziye understand what death state and lysis state actually look like. This was also a kind of new knowledge.

Truly, everywhere is learning.

Fang Ziye hurriedly switched to another, then another.

Found that all looked about the same, and Knowledge Points no longer increased.

"Too greedy, too greedy." Fang Ziye could only murmur so.

And while Fang Ziye was looking like this, he saw Luo Tingzhu had also prepared the various cell resuscitation reagents and instruments Fang Ziye had prepared earlier, and even carefully pre-warmed the PBS and culture medium in the 37°C constant temperature water bath first.

Striving to simulate normal human body temperature to the maximum extent, so the tumor cell babies could grow vigorously and happily after resuscitation, then become favorable experimental materials for Luo Tingzhu.

"Junior Sister Luo, should I help you throw these away?" Fang Ziye asked.

"Throw them away. I asked Senior Brother, contaminated like this, they can't be saved. I was still planning to do a scratch assay."

"Sigh~" Luo Tingzhu said a vocabulary that was a bit beyond Fang Ziye's current experimental progress.

Fang Ziye didn't delve into it immediately, but instead curiously glanced at his own cell culture dish again, discovering the cells were still in suspension, not having adhered yet, and felt he was too anxious.

After putting it back into the constant temperature cell incubator, Fang Ziye bid farewell to Luo Tingzhu.

Luo Tingzhu didn't need him to guide her in cell resuscitation anyway. Staying here would instead be like a joke, only adding pressure...

Fang Ziye had merely entered the lab, and was not in a full-detachment or half-detachment state. Therefore, clinical patients were still his responsibility. On Monday's surgery day, Fang Ziye still had to go see if there were any operating opportunities.

Monday, luck was pretty good.

Although Professor Deng Yong and Professor Xie Jinyuan were still pushing surgical progress relentlessly at full firepower, Fang Ziye's operating opportunities for his assigned patients were not stripped away.

And in the afternoon surgery, one station belonged to Fellow Trainee Jie Han. After splitting stations, Senior Brother Sun Shaoqing went to another station to operate. Therefore, Fang Ziye gained two opportunities to scrub in as an assistant.

The first station, Fang Ziye just acted as an assistant. He didn't touch a hair of the other operations; they were all gnawed away by Professor Deng Yong and his own Master.

Finally, after splitting stations, under his Master's feeding, Fang Ziye completed an incision technique first to preserve his hand feel.

Yuan Weihong's mood at this moment still remained on Fang Ziye bringing honor to the family. After watching, he said: "Entered the lab, but don't neglect clinical operations. Occasionally you must also go to the training room and the operating room."

Jie Han then said: "Master, hearing from other Senior Brothers, Senior Brother Fang was in the training room for two hours last night, didn't return until past eleven."

"Where did you hear so much from?" Yuan Weihong asked, turning his head.

Jie Han's eyes flickered rapidly a few times at this moment, replying: "Master, thanks to your good teaching, Senior Brother feeds me, I published a few more papers. So among the brothers, everyone gives face."

"There are also people asking me to teach them how to write a meta-analysis, but I didn't dare agree."

Nearly three-quarters of Fang Ziye's research output was done together with Jie Han. And Jie Han himself published one paper alone, and co-published one with Fang Ziye, already published. Another letter, also already published. One more revised paper, about to be published.

This research output can be described as fucking awesome.

And Jie Han himself also independently produced a meta-analysis, about to be finalized. Also heard that while Jie Han is soaking in the training room now, he collected some previous clinical data to do a retrospective clinical study analysis, currently also in the data collection process.

This research output is simply crazy.

How could one not love him?

"Virtue~"

"Any progress on suturing technique? If so, come suture a station?" Yuan Weihong looked at his two precious disciples, his mood relaxed as he raised an eyebrow.

"Master, still a little bit short. Just a little bit short and I can get the rubber skin approximated well. And incision technique isn't far off either." Jie Han replied so, as if reassuring Yuan Weihong that his research time hadn't delayed his clinical progress.

Yuan Weihong's mood instantly relaxed. He shouted loudly: "Circulating Teacher, Anesthesiologist!"

The anesthesiologist was playing on his phone. Yelled at by Yuan Weihong, his whole body trembled. Subconsciously checking vital signs, all stable. He also loudly replied in a heavy Northeast accent, glaring angrily: "What are you doing?"

"Eat together tonight!" Yuan Weihong continued loudly.

"Eat eat, why are you so loud?" The anesthesiologist's voice lowered.

But immediately, he put his phone away, lowered his voice: "Wei Ge, where are we going to eat?"

Yuan Weihong performed surgery while discussing where to eat with the circulating nurse and anesthesiologist.

Jie Han carefully pondered the details of Yuan Weihong's operation on the surgical table, one hand helping while the other flexibly shifted, as if pondering.

Fang Ziye felt the Shadowless Lamp's light didn't seem that strong anymore, feeling an unprecedented sense of ease.

The main thing was, when the surgery ended.

"Knowledge Points +0.4" floated, making Fang Ziye's mood increasingly relaxed.

This book's rhythm is daily life plus surgery, mixed with outpatient clinic and laboratory; please readers take note, there will occasionally be big satisfaction points, but in most situations, it's this rhythm and progress.

Everyone don't rush, slow work yields fine product; Xiao Yu strives for every word coded out to let everyone read a bit more relaxed.

Remind again, the research pathways in this text, don't correspond with reality, thanks; also don't ask Author this already done, this impossible stuff, Author impossible to publish some pathways self done.

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(Chapter End)