Chapter 40: Charging Forward Without Regard for Anything (Asking for Favorites, Asking for Follows)

If we're talking about the medical field, how do you make research personnel angry?

There are a few good ways to do it.

First, you cuckold him.

Second, you contaminate the cell line he's culturing.

Third, you give a cat to the mouse he's raising.

You'll be able to see him go on an ultimate rampage in all cases.

Cell lines, or cell systems, are the most fundamental and also the most core form of accumulation and inheritance for a medical research worker.

Some processed cell lines aren't something you can just buy whenever you want; purchased cell lines are generally unprocessed and not suitable for the research group.

It's like this: if the cell line you buy is raw silk, then a mature cell line is already woven cloth, just waiting to be made into clothes.

In the cell room, Fang Ziye disinfected experimental supplies with UV light on one hand, while placing PBS buffer and aliquoted culture medium into a 37-degree constant temperature water bath to warm up on the other.

At the same time, he expounded the basic cell experiment process to his Master: "Master, the cell scratch assay is an experimental method to investigate cell migration ability, repair ability, and mutual interaction."

"It mainly simulates cell migration. Also called cell movement, cell crawling, or cell motility, it refers to the movement produced after cells receive migration signals or sense gradients of certain substances."

"Cell migration participates in many physiological and pathological activities, such as immunity, inflammation, tumor metastasis, injury repair, and embryonic development."

"The cell scratch assay is a laboratory technique that studies cell migration motility, repair ability, and intercellular interaction by creating a scratch on a cell monolayer and periodically capturing images through time-lapse microscopy..."

"The experimental steps are as follows." Fang Ziye reported them one by one, and reported them very rigorously.

After a full ten-plus minutes, Yuan Weihong finally nodded slightly: "Understanding this much, you don't count as a disaster anymore."

"Let me see your lab notebook." Yuan Weihong took the lab notebook Fang Ziye bought online, tapped it, and said: "This thing, all its contents, you'll have to transcribe into this lab's experimental data record book later."

"You go get one?"

"Master, isn't it because I just arrived and don't dare go get one yet? Making a draft first isn't bad either." Fang Ziye knew that when doing experiments, the original experimental data, including experimental protocols, all need to be preserved.

This is the most important evidence for publishing papers later, and even for project completion, proving you actually did the experiments rather than randomly making things up. Some top journals require these as supporting materials when accepting papers to prevent academic fraud.

But Fang Ziye wouldn't dare act recklessly in Professor Deng Yong's lab; he hasn't even officially entered yet.

Lab notebooks aren't something you can just get by asking; Luo Tingzhu hasn't gotten one either.

"Then record it well first. After all the scratch assay data is recorded, write an application and hand it to Professor Deng; he'll give you one." Yuan Weihong had also come up through being a small Master's student and small Doctor, so he knew Fang Ziye's inner apprehensions.

After checking, Fang Ziye had written the time, steps, and protocols quite well one by one, with no mistakes or omissions, so he was reassured.

Unfortunately, though, Fang Ziye's cultured cells still haven't grown to cover the entire culture dish.

So right now it doesn't count as completing cultivation and passaging, and freezing and other operations can't be completed yet.

Of course, Fang Ziye still completed the cell transfer experimental operations one by one under Yuan Weihong's personal witness, very proficiently.

Cell transfer is actually just the operation of transferring cells from one culture dish to another, to avoid cells staying in one dish too long and developing contamination.

This is also one of the basic operations of cell experiments.

It's equivalent to little kids in kindergarten sleeping pressed against each other — you're against me, I'm against you! Trypsin digestion is waking up all these little friends, then randomly dividing them into several classes, letting these little friends return to classrooms in certain quantities, and sleeping again!

Trypsin digestion makes adherent cells suspend — that's the process of waking up the little friends.

"Not bad, you prepared beforehand, right? Coveting a PhD for a long time, huh." Yuan Weihong deliberately drew out his tone, gratified but inevitably a bit smug.

A student's progress naturally makes a teacher happy.

Fang Ziye chuckled, neither refuting nor explaining.

At this stage, who doesn't want to do a PhD? Who doesn't want to stay at a major hospital?

Yuan Weihong walked out first with hands behind his back, waiting for Fang Ziye to finish processing the experimental equipment, return the reagents that belonged in the refrigerator, and return the experimental tools that belonged in the storage cabinet to their places one by one.

Continuing with hands behind his back, he led this little chick Fang Ziye toward the lab exit, saying: "Giant cell tumor of bone is a borderline tumor; although it's not as hot as osteosarcoma."

"But publishing papers isn't about the hotter the better; if what you do is deep enough, you can publish high-impact papers."

"Journal editorial departments mainly look at your paper's experimental content, not whether it's hot enough. So don't feel that because you're culturing giant cell tumor, it's a grade lower than someone else's osteosarcoma; it's not like that."

Fang Ziye nodded: "Master, I naturally know. The most important thing is mastering experimental methods, filling the current unknown gaps in academia — that's what most research teams are doing, not making breakthroughs."

"The most important thing is mastering and learning methods. With methods, there are so many unknown blanks in academia now; randomly find a gap and plug it, and that's academia!"

Today's transfer is complete. Although knowledge points didn't increase as much as the previous few times, there's still 0.5.

Fang Ziye is very much looking forward to the knowledge point growth speed the subsequent scratch assay can bring.

"Good that you have this understanding. I can't treat you to a meal today; I have to go eat at my father-in-law's with your Master's wife. You guys figure it out yourselves."

"When you return from the training room in the evening, come back earlier, and pay attention to balancing work and rest."

"Oh, right. I heard from people in the lab that Luo Tingzhu, she's been pushing herself really hard lately? Do you know what's going on?" Yuan Weihong suddenly flashed a look, asking like this.

Fang Ziye shook his head: "Junior Sister Luo is pushing hard?"

Fang Ziye really hadn't noticed.

"She usually stays in clinical until afternoon, in the training room until evening, then in the lab until early morning. The next day's ward rounds, she still arrives on time. This little girl doesn't cherish her body at all."

"Don't know why she's pushing so hard. You're the same as others — keep an eye out if you see her, don't let her faint."

"Our Orthopedics originally has very few girls. Our Trauma Surgery has just this one, Sports Medicine one, Joint Surgery one. They still need a bit of protection."

"Girls can't take as much punishment as boys." Yuan Weihong said.

"Okay! I'll pay attention, Master." Fang Ziye's heart tightened.

In this major hospital, who isn't struggling hard? Simple and fulfilling, or not simple but still fulfilling.

(Chapter End)