Chapter 41: There Must Be an Entrustment (Seeking Collections, Seeking Continued Reading)
Doing experiments is like performing surgery: both require extra rigor, thorough preparation, control of relevant factors, and avoidance of mistakes.
Even in some cases, variable control in experiments is more extreme than in clinical practice.
To do a scratch assay and prove which cell baby runs faster is far harder than making a baby, where you only give one shudder and then let it develop freely!
Everyone must be fully prepared, with all possible influencing variables controlled.
First, everyone must be on the same line, with the scratch width identical.
Second, all three cell lines must be fully grown; otherwise, if one cell line is few and another is numerous, and you claim it runs faster, the journal editor will reject your manuscript outright, politely telling you to scram, and your tantrums will be useless.
Moreover, the three cell lines grow at different speeds, so even if cultured for the same time, one may be old and dead while the other hasn't reached adulthood—concubine-born before the lord is born, lord born before the concubine is old.
A four-year-old boy and a fifty-year-old unmarried woman on a blind date—how could they get along? Thus the start times of culture must be staggered, ideally letting different cell lines be in their proper prime of seventeen-eight or twenty-one-two, for convincing preparation.
Purchasing labeling reagents, equipment, and so on must all be complete, and preparations must be made for repeating the experiment if it fails; thus it is painstaking work requiring careful thought and slow preparation to prove through visualized data who runs faster.
Fang Ziye expected that about ten days later, around the twenty-third or twenty-fourth, the test would officially begin, which was still about...
Before that, he should first passage several generations of cells and freeze more surplus material in the liquid nitrogen tank—better safe than sorry; experimental steps are not limited to the scratch assay alone.
On Thursday, Fang Ziye still devoted most of his time to clinical work, carefully refining treatment plans for his patients, not to truly correct medication types, but to read more literature and improve his understanding—that was the right approach.
Through several months of reading, organizing, and summarizing, although Fang Ziye had never added knowledge points to basic theory, his basic theory had still improved somewhat.
【Medical Basic Theory: Surgery Level 2 15/50, Orthopedics Level 2 22/50, Trauma Surgery Level 2 35/50...】
【Specialty Basic Theory: Postoperative Medication Experience in Trauma Surgery Level 2 45/50, Infection-related Medication Experience 33/50, Perioperative Diagnosis and Treatment Experience 37/50...】
Medical Basic Theory is what all medical students must learn, including internal medicine, surgery, and medical comprehensive (dermatology, psychiatry, neurology, infectious diseases), etc.
However, Fang Ziye's depth in these medical basic theories was naturally insufficient—where would a surgeon find so much time for these theories?
Specialty Basic Theory, on the other hand, mainly targets theoretical reserves beyond surgical treatment. Although surgery is the bulk of surgical treatment, it is by no means entirely surgical.
Through summarizing and induction, Fang Ziye derived a rule: namely, Surgery, Orthopedics, and Trauma Surgery in Medical Basic Theory are respectively related to diagnostic ability and cognitive depth regarding common diseases in Surgery, Orthopedics Department, and Trauma Surgery.
Diagnosis is easy to understand, but cognitive depth is another category—preoperative.
Specialty Basic Theory mainly concerns perioperative and postoperative nursing, such as judging whether there is infection, and the pros and cons of surgical outcomes.
Is medicine really that simple? Is everything over once the surgery is done?
Of course, these efforts are even harder to see and harder for people to discover; unlike surgical operations, where whether you are dexterous and exquisite is obvious at a glance.
But one must still slowly ponder through the patients under one's charge: why guidelines are formulated this way, why medications are used this way, so that if similar problems arise in the future, one knows how to solve them.
During the Master's and Doctoral Student period, if one can solidify Medical Basic Theory and Specialty Basic Theory, only then can one possess the weighty knowledge reserve to gradually perform basic medical operations and take the lead as Lead Surgeon, handling unexpected situations when they arise.
Otherwise, if your understanding is insufficient, how can you handle unexpected situations, or cases that don't follow the textbook?
Naturally, this process was not done by Fang Ziye alone; Luo Tingzhu and Deng Yong's Group's First-year Doctoral Student Senior Brother Yan Zhiming were doing it too.
Luo Tingzhu and Yan Zhiming were both about to take over as Follow-up Resident; although Follow-up Resident, they were actually Deng Yong's Group's Chief Resident, in charge of all matters within the group, and needed to shoulder the heavy burden.
After that was drilling in the Training Room.
Time flows like water; in a blink, another three days passed.
On Friday Surgery Day, Shaoqing Senior Brother still occupied surgical resources, causing Fang Ziye's Friday knowledge point farming plan to fall through; he could only gain a pitiful few knowledge points through reading and staying in the Training Room.
After adding points to X-ray Reading Technique last time, Fang Ziye's knowledge points had not yet broken into double digits.
Fang Ziye also knew that improving theory and diagnostic ability was better, but knowledge points were simply insufficient; with two Surgery Days and one Outpatient Day per week, would you say improving professional operation ability is better, or CT and MRI image reading level?
If he could cut into a large lesion or something, then when the time came he himself wouldn't be able to get on stage—wouldn't that be missing a great opportunity?
As for the Outpatient Clinic, after all, it was his own Master; Master delegated power, slowly letting go—it was natural progression. Showing one's ability in the operating room requires being able to take it on all at once, and take it well, to have more opportunities.
Fang Ziye finished practicing screw practice in the Training Room. Actually it was the screw removal and placement in fracture open reduction internal fixation surgery, also a kind of operation, simple, with no requirements.
But Fang Ziye was preparing for the possibility that Teacher or someone might accidentally admit a patient for internal fixation removal surgery, in which case wouldn't he have the opportunity to independently serve as Lead Surgeon?
Then he stopped to watch Luo Tingzhu practice the more advanced incision technique on pork dissection material; after operating, there was no doubt it was a failure, not meeting the standard.
Dissecting dead pork was actually not as easy to dissect as carving characters into tofu.
"Junior Sister Luo, are you going to the Laboratory later?" Today was Saturday, and the time was still early, only eight in the evening.
"Going, I booked the Cell Room for ten tonight. Senior Brother, aren't you going today?" Luo Tingzhu planned to cut open once more, so she took another lump of pork with skin, slightly smaller than a fist, and smashed it onto the operating table, like a female butcher.
Luo Tingzhu also knew that after Fang Ziye entered the Laboratory, he was about as diligent as her, so she turned her head and asked with a smile.
"Rest today; I just changed media yesterday, and the Normal Group cell line isn't needed until tomorrow."
"Junior Sister Luo, you should rest too; how long has it been since you properly rested?" Fang Ziye mentioned casually.
Luo Tingzhu turned back, picked up the Willow Leaf Knife and sliced down, saying: "I have no way; I'm still doing preliminary research basics for grant applications now; only after getting the grant will there be money for paper content."
"The General Project and Provincial Level 1 Project applications are also about to start. I want to submit a school-level project grant myself, and also submit two in Teacher Deng's name..."
Hearing this, Fang Ziye smiled and said: "Junior Sister Luo, haven't you graduated enough already?"
"But I still need to find a job; if I don't properly do experiments and write papers now, at most I can only stay in this hospital's Emergency Department and not enter the specialty." Luo Tingzhu turned back, then continued carefully dissecting the pork.
Fang Ziye didn't persuade her anymore.
What enables a person to harbor such a profound obsession must have an anchor; what is anchored is never lost, and thus the obsession is impossible to resolve.
(Chapter End)
End of Chapter
